Main topics of the Research Laboratory of the Children's Hospital

Utilisation of ¹³C-labelled glycosyl ureides for oro-caecal transit time measurements
Fate of ingested doubly labelled probiotica
Impact of L-carnitine on fat oxidation
Effect of alcohol on liver detoxication capacity
Gastric emptying
Effect of resistant starch on colon metabolism and fat oxidation

PDF Lecture : "Stable isotopes in gastroenterology and nutrition"

International scientific activities

Co-operation partners:
Prof. Dr. Jörg Dötsch
University Hospital Erlangen
Department of Pediatrics

Dr. Frans Stellaard
University Medical Center Groningen
Department of Laboratory Medicine

BASIS-member (Benelux Association of Stable Isotope Scientists)

Member of the GASIR (German Association for Stable Isotope Research)

Member of the Editorial Board of Isotopes in Environmental and Health Studies

Publications 2004-2009

Book chapters, proceedings and monographs

Heine W, Mohr C, Wutzke KD:
Glykosylureide, Abschlussbericht.
DFG-Projekt: He 2058/2-1/2-2, 1997.

Peer reviewed papers

Wutzke KD, Glasenapp B:
The use of ¹³C-labelled glycosyl ureides for evaluation of oro-caecal transit time.
Eur J Clin Nutr 2004;58:568-72.
Wigger M, Wutzke KD:
Whole-body protein turnover in nephrotic subjects: a comparison between different degrees of proteinuria.
Isotopes Environ Health Stud 2004;40:155-61.
Wutzke KD, Lorenz H:
The effect of L-carnitine on fat oxidation, protein turnover and body compostion in slightly obese subjects.
Metabolism 2004;53:1002-6.
Wutzke KD, Oetjens I:
¹³C- and ¹⁵N-incorporation of doubly stable isotope labelled Lactobacillus johnsonii in humans.
Eur J Clin Nutr 2005;59:1167-72.
Wutzke KD, Sattinger V:
¹⁵N-excretion of heat-killed Lactobacillus casei in humans.
Eur J Clin Nutr 2006;60:847-52.
Wutzke KD, Schütt M:
The duration of enzyme induction in orocaecal transit time measurements.
Eur J Clin Nutr 2007;61:1162-6.
Schönhusen U, Kuhla S, Zitnan R, Wutzke KD, Huber K, Moors S, Voigt J:
Effect of a soy protein-based diet on ribonucleic acid metabolism in the small intestinal mucosa of goat kids.
J Dairy Sci 2007;90:2404-12.
Götz KP, Staroske N, Radchuk R, Emery RJ, Wutzke KD, Herzog H, Weber H:
Uptake and allocation of carbon and nitrogen in Vicia narbonensis plants with increased seed sink strength achieved by seed-specific expression of an amino acid permease.
J Exp Bot 2007;58:3183-95.
Wutzke KD, Forberger A, Wigger M:
Effect of alcohol consumption on the liver detoxification capacity as measured by [¹³C]methacetin- and [methyl-¹³C]methionine-breath tests.
Isotopes Environ Health Stud 2008;44:219-26.
Wutzke KD, Berg D, Haffner D:
The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans.
Eur J Clin Nutr 2008;62:197-202.
Wutzke KD, Wigger M:
Effect of alcohol consumption on the liver detoxication capacity as
measured by [13C2]aminopyrine- and L-[1-13C]phenylalanine-breath tests.
Isotopes Environ Health Stud 2009;45:185-91.
Wutzke KD, Lotz M, Zipprich C:
The effect of pre- and probiotics on the colonic ammonia metabolism in man as measured by the biomarker lactose-[15N2]ureide.
Eur J Clin Nutr 2010, in review.
Wutzke KD, Mix J:
The metabolic fate of doubly labelled lactose-[13C, 15N]ureide after pre-dosing with different ureides.
Eur J Clin Nutr 2010, in preparation.

Impact points 2004-2009: 14.55

Patents

Plath C, Russow R, Sich I, v Bismarck P, Wutzke KD:
Verfahren zur Bestimmung der [¹⁵N]Stickstoffmonoxid-Exhalation zur Diagnostik und Therapie bei Erkrankungen des Menschens.
DE 197 19 098 C1;1999, IPC: A61K 49/00

Dissertations and third-party funds of the Research Laboratory

Björn Glasenapp
Utilisation of glycosyl ureides for measuring oro-caecal transit time (OCTT).
(Scientific Award of the GPGE, Potsdam 2002)
Defense of doctoral theses 2002: magna cum laude
Frederick Palm
The impact of L-carnitine on fat oxidation.
Defense of doctoral theses 2003: cum laude
(in cooperation with Hero Ltd. Lenzburg, Switzerland)
Daniela Gohr
The effect of lactose ureide dosage on OCTT measurement.
Defense of doctoral theses 2003: cum laude
Kirstin Händler
The nutritional effect on gastric emptying and OCTT.
Defense of doctoral theses 2004: cum laude
Iris Oetjens
Utilisation of doubly labelled probiotic Lactobacillus acidophilus (LC₁).
(Grant of the Karl and Veronica Carstens-Foundation)
Defense of doctoral theses 2004: magna cum laude
Manja Schütt
The duration of enzyme induction by lactose ureide on OCTT measurement.
Defense of doctoral theses 2005: cum laude
Henrik Lorenz
The impact of L-carnitine on fat oxidation and protein turnover in slightly obese subjects.
Defense of doctoral theses 2005: magna cum laude
(in cooperation with Lonza Ltd., Basle)
Verena Sattinger
Utilisation of killed ¹⁵N-labelled L. casei.
(Grant of the East Tennessee State University)
Defense of doctoral theses 2005: magna cum laude
Dominika Berg
Utilisation of killed, doubly labelled LC₁.
Defense of doctoral theses 2006: cum laude
Anke Meinke
The impact of alcohol on detoxication capacity of the liver measured by [¹³C]methacetin-
and [methyl-¹³C]methionine-breath tests.
Defense of doctoral theses 2006: magna cum laude
Susanne Müller (Prof. Wigger)
The effect of L-carnitine on fat oxidation in nephrotic syndrome.
Defense of doctoral theses 2006: cum laude
Christian Gereke
The impact of L-carnitine on glucose oxidation.
Defense of doctoral theses 2007: cum laude
(in cooperation with Lonza Ltd., Basle)
Juliane Haase (Prof. Plath)
The influence of complementary food on gastric emptying and OCTT in infants.
Defense of doctoral theses 2008: cum laude
(in cooperation with HiPP, Pfaffenhofen)
Martin Dürr
The effect of L-carnitine dosage on gastric emptying and OCTT.
Defense of doctoral theses 2008: cum laude
Carolin Griesheim
The effect of L-carnitine on fat oxidation in elderly subjects.
Defense of doctoral theses 2008: cum laude
(in cooperation with Lonza Ltd., Basle)
Tina Teichmann (Prof. Wigger)
The effect of cyclosporine A on fat oxidation of transplanted subjects.
Defense of doctoral theses 2008: cum laude
Susanne Staar
...[methyl-13C]ethanol and [1-13C]galactose-breath tests.
Defense of doctoral theses 2009: magna cum laude
Ramona Dummer
The effect of growth hormon therapy on fat oxidation.
Defese of the doctoral theses 2009: cum laude
(in cooperation with Pharmacia, Erlangen)
Rebekka Wichmann
Excretion and incorporation of orally administered 15N-labelled yeast nucleic acids in healthy adults.
Defese of the doctoral theses 2009: cum laude
Johanna Mix
The metabolic fate of doubly labelled lactose-[¹³C, ¹⁵N]ureide after pre-dosing with different ureides.
(in cooperation with Campro Scientific, Berlin)
Andrea Popp
The effect of L-carnitine supplementation on fat oxidation, respiratory quotient, and protein turnover in adipose subjects.
(in cooperation with Lonza Ltd., Basle)
Carina Zipprich
Lactose-[¹⁵N₂]ureide: a biomarker for assessing pro- and prebiotic effects in humans.
(in cooperation with Emsland Group GmbH, Emlichheim)
Bernhard Zimmermann
The impact of L-carnitine on fat oxidation, respiratory quotient, and protein turnover in adipose subjects.
(Ph.D. theses, FORUN-project, in cooperation with Lonza Ltd., Basle)
Steffen Büchner
The effect of an L-carnitine/ omega-3 fatty acid mixture on fat oxidation in obese subjects.
(in cooperation with STADA, Bad Vibel)
Kay Haase
...[1-¹³C]ketoisocaproic acid and [methyl-¹³C₃]caffeine-breath tests.
Gerrit Bruns
The impact of alcohol consumption on protein turnover in men measured by using ¹⁵N-labelled yeast protein.
Silvia Nern
The impact of cigarette smoking and caffeine on detoxication capacity of the liver
measured by [¹³C₂]aminopyrine and [¹³C₃]caffeine-breath tests.
Katharina Roost
Effect of resistant starch on fat oxidation in humans.
Antje Krzyzanowski (PD Dr. Walther)
OCTT measurements in healthy children and in children suffering from chronic inflammatory bowel diseases.
N.n.
Utilisation of ¹⁵N-labelled L. casei.
Jennifer Trajera
...[methyl ¹³C₂]aminopyrine- and [1-¹³C]phenylalanine-breath tests.
Erik Heindl
The impact of alcohol consumption on gastric emptying and OCTT in men.
Steffen Harder
Evaluation of two different pancreatic enzyme preparations in cystic fibrosis by using a tripalmitate-trioleate ¹³CO₂-breath test.